Plasmid_Backbone
BKD

Part:BBa_K4074044

Designed by: MIT iGEM 2021   Group: iGEM21_MIT   (2021-10-01)


pSB_BKD

Contents: BKD operon
What is it?: A basic part - plasmid backbone
What does it do?: It codes for bkd operon homology arms, which allows for integration into the B. subtilis genome at the bkd operon.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2209
    Illegal XbaI site found at 2182
    Illegal PstI site found at 2170
    Illegal PstI site found at 3364
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2209
    Illegal PstI site found at 2170
    Illegal PstI site found at 3364
    Illegal NotI site found at 3357
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2209
    Illegal BamHI site found at 2188
    Illegal XhoI site found at 171
    Illegal XhoI site found at 1063
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2209
    Illegal XbaI site found at 2182
    Illegal PstI site found at 2170
    Illegal PstI site found at 3364
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2209
    Illegal XbaI site found at 2182
    Illegal PstI site found at 2170
    Illegal PstI site found at 3364
    Illegal NgoMIV site found at 3328
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


[edit]
Categories
Parameters
None