Plasmid_Backbone
BKD
Part:BBa_K4074044
Designed by: MIT iGEM 2021 Group: iGEM21_MIT (2021-10-01)
pSB_BKD
Contents: BKD operon
What is it?: A basic part - plasmid backbone
What does it do?: It codes for bkd operon homology arms, which allows for integration into the B. subtilis genome at the bkd operon.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2209
Illegal XbaI site found at 2182
Illegal PstI site found at 2170
Illegal PstI site found at 3364 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2209
Illegal PstI site found at 2170
Illegal PstI site found at 3364
Illegal NotI site found at 3357 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2209
Illegal BamHI site found at 2188
Illegal XhoI site found at 171
Illegal XhoI site found at 1063 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2209
Illegal XbaI site found at 2182
Illegal PstI site found at 2170
Illegal PstI site found at 3364 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2209
Illegal XbaI site found at 2182
Illegal PstI site found at 2170
Illegal PstI site found at 3364
Illegal NgoMIV site found at 3328 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
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Categories
Parameters
None |